Characterization of bituminite in Kimmeridge Clay by confocal laser scanning and atomic force microscopy
نویسندگان
چکیده
This work investigates bituminite (amorphous sedimentary organic matter) in Upper Jurassic Kimmeridge Clay source rock via confocal laser scanning microscopy (CLSM) and atomic force (AFM). These petrographic tools were used to provide better understanding of the nature bituminite, which has been historically difficult identify differentiate from similar matter types rocks. As part an International Committee for Coal Organic Petrology (ICCP) working group, immature (0.42% vitrinite reflectance), organic-rich (44.1 wt% total carbon content) sample was distributed multiple laboratories CLSM characterization. The primary observations imaging spectroscopy include: 1) interpreted presence Botryococcus algae as a contributor precursors; 2) color red-shift sulfide reflectance auto-fluorescence below surface; 3) positive alteration laser-induced photo-oxidation surface, including fluorescence blue-shift; 4) blue-shift associated higher intensity regions indicative compositional (fluorophore) differences; 5) need standardization applied CLSM; 6) suitability predict solid bitumen spectral emission calibration extant dataset. Secondary include detection reflected light highly reflective inclusions sulfides fusinite, radiolytic caused by substitution U Fe sulfides. Findings AFM observation that surface roughening or flattening are induced differential broad ion beam (BIB) milling dependent on location scale topology measurement. result highlights our still limited effects BIB indicates further research before this technique can be advanced standard practice preparation. Collectively, results study illustrate general applicability versatility petrology research, specifically properties maceral.
منابع مشابه
Combining Atomic Force Microscopy and Laser Scanning Confocal Microscopy
Introduction The visualisation of sample elements using various forms of microscopy relies not only on magnification but also contrast. As such, differing forms of microscopy offer different information about a sample. Laser scanning confocal microscopy (LSCM) provides information about the 3D location of a particular, labelled component within a sample and has the additional advantage of exclu...
متن کاملLaser Scanning Confocal Microscopy
Laser scanning confocal microscopy has become an invaluable tool for a wide range of investigations in the biological and medical sciences for imaging thin optical sections in living and fixed specimens ranging in thickness up to 100 micrometers. Modern instruments are equipped with 3-5 laser systems controlled by high-speed acousto-optic tunable filters (AOTFs), which allow very precise regula...
متن کاملConfocal laser scanning microscopy.
Many technological advancements of the past decade have contributed to improvements in the photon efficiency of the confocal laser scanning microscope (CLSM). The resolution of images from the new generation of CLSMs is approaching that achieved by the microscope itself because of continued development in digital imaging methods, laser technology and the availability of brighter and more photos...
متن کاملCombining Atomic Force Microscopy with Confocal Microscopy
Confocal fluorescence microscopy and atomic force microscopy (AFM) are two established techniques that can be used to study single molecules. Both techniques have their individual capabilities that permit to study different aspects such as topography using AFMs, or dynamics using fluorescence spectroscopy. Thus, a combination of both techniques into a single instrument enhances the methodology ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: International Journal of Coal Geology
سال: 2022
ISSN: ['0166-5162', '1872-7840']
DOI: https://doi.org/10.1016/j.coal.2022.103927